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FDA Standard Next Gen
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FDA Standard Sequencing
FDA Standard QPCR
FDA Standard Microarray
FDA Standard Microbial ID
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Research Services

QCPR

 

FDA Level QPCR

We are an Applied Biosytems TaqMan® Authorized Service Provider

Real-time PCR remains one of the most sensitive and quantitative tools for gene expression used today. SeqWright offers both custom and pre-optimized QPCR assays for an assortment of specific applications. We manage all aspects of the project from assay design, to nucleic acid isolation, to data analysis

Quantitative PCR Services

  • Copy number determination of DNA or RNA targets
  • Allelic discrimination / SNP genotyping
  • Gene expression profiling (siRNA knockdown & microarray study validation)
  • microRNA analysis
  • Plus/minus assays
  • Microbial detection and residual DNA analysis
  • Biodistribution studies of gene therapy products
  • Transgenic mouse genotyping

Service Features and Options

  • QPCR primer and probe design
  • Nucleic acid isolation from tissue, cells, biotechnology products, etc.
  • Quantitation using a NanoDrop® ND-1000
  • Qualitative analysis by an Agilent BioAnalyzer®
  • Primer/Probe optimization
  • Samples assayed in duplicate to ensure reproducibility
  • Controls included on each thermocycling run
  • Data report with project design description
  • Statistical analysis upon request

Disease Modeling

SeqWright provides a wide variety of assays for studying disease pathways. These assays take advantage of TaqMan® Gene Signature Arrays which contain TaqMan Gene Expression Assays matching genes specific to disease target classes or pathways. The genes are chosen from pathway analysis tools, published articles, and collaborators input.

Click here to see a list of available TaqMan® Gene Signature Arrays.

High-throughput Meets Customization

Utilizing ABI’s 7900HT and TaqMan® 384-well micro fluidic cards, SeqWright has the capability of running hundreds of real-time PCR reactions simultaneously. We require only small amounts of sample, to run 1 to 8 samples in parallel against 12 to 384 TaqMan® Gene Expression Assay targets.

We can help you choose and design custom TaqMan® Arrays using any of ABI’s 50,000 inventoried TaqMan Gene Expression Assays and nine array formats. Important note: depending upon the format, ABI requires certain numbers of arrays be purchased when manufacturing customized cards.

Click here to learn more about TaqMan 384-well micro fluidic cards.

microRNA Services

From isolation of small RNA’s to downstream analysis of their expression level, just send us your samples and we can do the rest. miRNA's offer insight into a complex world of gene regulation that is just beginning to come to light. Let our RNA Scientists and Bioinformatic experts help you get the most out of your miRNA experiments.

SeqWright can perform microRNA Analysis on the following Arrays

Biodistribution Studies

Biodistribution analysis is a critical tool for measuring the efficiency and distribution of various gene therapy expression vectors or unwanted viral/nucleic acid contamination within normal and diseased tissue. Quantitative PCR represents one of the most reliable and sensitive tools for accessing the presence and level of DNA or RNA within both the target and surrounding tissue. SeqWright can design, optimize and validate custom Biodistribution assays for you, or run an assay of your choice. Once an assay is designed, SeqWright will be available to test your samples as needed and provide a comprehensive report, including the necessary statistical analysis. Let SeqWright's qPCR experts design and implement your Biodistribution studies and you can be confident in the efficiency and specificity of your expression vectors.  

Additional Resources