Clinical/GLP Shotgun Library Construction and Sequencing
Shotgun sequencing is a method of sequencing very large DNA fragments/genomes by random DNA strand fragmentation, fragment cloning and sequencing with overlapping clone sequences aligned to produce a complete sequence.
In the industry, SeqWright has a distinct advantage in Shotgun library construction and sequencing as it is the exclusive supplier of custom shotgun libraries prepared by the 'Double Adaptor' method, utilized by the Baylor College of Medicine – Human Genome Sequencing Center in completing the Human Genome Project.
Regulated Shotgun Service Features
- Double adapter shotgun library construction.
- Plasmid pWright™ vector capable of holding inserts up to 10kb (typically 1-3kb).
- High percentage of clones carry inserts.
- No chimerics.
- Libraries submitted to customer as plates, glycerol stocks, or purified DNA.
- DNA sequencing Quality Control to ensure clones carry inserts.
- 2 week turnaround time in most cases.
Regulated Whole Genome Shotgun Sequencing
- FDA GLP sequencing.
- Automated colony picking and arraying (96- or 384-well plate format).
- Automated DNA preparation.
- High-throughput sequencing using ABI Prism™ 3730xl sequencers.
- Contig assembly, mapping and gap closure.
- LIMS real-time tracking.
More Regulated Library Construction Options
Additional Regulatory Services
- Custom plasmid vector libraries construction.
- RNA or gDNA preparation.
- Automated DNA/RNA extraction.
- cDNA library construction.
Additional Resources
- Available Genome annotation (Pfam/CoG/BLAST assignments) service.
- Project specific Bioinformatics solutions.
- Contact a sales representative about our services.
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