SeqWright -Next Gen DNA Sequencing

	Next Generation Sample Preparation Guidelines The following guidelines are designed to give you a basic idea of how to prepare your samples prior to sending us your next generation project. By following these guidelines, you will help us produce the best possible quantity and quality of data for you. However, not every application can be covered here and each project is different so, please contact us for your free project-specific criteria.
	GS FLX Titanium Sample Preparation
	Fragment Library-based Projects: DNA must be double-stranded Fragments must be > 70 bp If fragments are 800bp or longer, we require a minimum of 10µg of DNA For fragments less than 800bp, we require a minimum of 2µg of DNA DNA should have an OD260/280 ratio of 1.8 or more DNA concentration should be a minimum of 50ng/ µl in TE buffer DNA should not be degraded, nor should it contain any particulate matter Paired-end Library-based Projects: DNA must be double-stranded Input DNA should be >10kb Minimum of 10µg of DNA is required DNA should have an OD260/280 ratio of 1.8 or more DNA concentration should be a minimum of 50ng/ µl in TE buffer DNA should not be degraded, nor should it contain any particulate matter Amplicon Library-based Projects: : DNA must be double-stranded Input DNA should be < 800bp long Minimum of 500ng of DNA is required DNA should have an OD260/280 ratio of 1.8 or more DNA concentration should be a minimum of 50ng/ µl in TE buffer DNA should not be degraded, nor should it contain any particulate matter
	If you are unable to meet these sample requirements, please contact a SeqWright representative for suggestions and technical support.

Next Generation Sample Preparation Guidelines