Sequence Capture Arrays and Genomic Enrichment
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| Agilent Technologies - SureSelect | ||
As a Certified Service Provider of the SureSelect Target Enrichment System, Seqwright has obtained the ability to improve the cost- and process-efficiency of next-generation sequencing. Based on an extremely efficient hybrid selection technique, SureSelect can be automated and easily scaled to meet the needs of larger sequencing projects. This is unlike any other commercially available target enrichment method. With sample input requirements at or below 3 µg of gDNA, researchers can perform highly targeted next-generation sequencing on the most precious of samples. |
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| NimbleGen | ||
As an Authorized Service Provider for NimbleGen Sequence Capture Arrays, SeqWright can utilize this technology in concert with its next generation genomics capabilities to capture, amplify and sequence up to 30MB per 2.1M array of client-specified gDNA from the human (hg18) genome. The captured DNA can be derived from one contiguous region of the genome or from thousands of non-contiguous segments (>250bp). This technology enables customized genomic enrichment and subsequent analysis which results in significant decreases in time and expense while maintaining quality and sensitivity. There are two options for custom arrays: the 2.1M custom array which captures up to 30MB of sequence and the 385K custom array which captures up to 5MB of sequence. |
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| NimbleGen - 2.1M Human Exome Array | ||
The 2.1M Human Exome Array contains 2.1 million long-oligo features which enables the entire human exome, all 180,000 exons, to be captured on a single array. The array captures some 550 miRNA's as well. These arrays are pre-designed, validated and ready to go which means the turnaround time from sample preparation, to enrichment, to next generation sequencing only takes a matter of weeks. That is very significant considering complete exonic amplification and sequencing may have taken a year or more prior to the development of this technology. |
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| RainDance Technologies - RainStorm™ | ||
| SeqWright has also recently begun providing RDT’s innovative RainStorm™ microdroplet-based technology to furnish researchers with another method of maximizing the efficiency of next-generation sequencing technology. This technology produces picoliter-volume droplets at a rate of 10 million per hour, avoiding unnecessarily complex automation solutions. Each droplet is the functional equivalent of an individual test tube and can contain a single molecule, reaction, or cell. The droplets are processed on a disposable chip that has no moving parts or valves. Samples have minimal contact with either walls or air. When this versatile technology is applied to highly-cited assays,high-speed workflows are created that minimize the process-induced bias or error commonly associated with multiplexed technologies. With its preparative and analytical capabilities, RainStorm technology is sufficiently flexible for a variety of applications using a simplified workflow integration of sample preparation, analysis, on-chip processing, and sample recovery for subsequent investigations. | ||
| Applications | ||
The applications of sequence capture array, targeted enrichment and microfluidic technology combined with next generation genomics are as broad as they are powerful. They enable the average researcher to take on projects from multifactorial diseases studies to the analysis of multiple gene sets involved in specific metabolic pathways. General applications of these technologies include: |
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| Learn more about the capture array process and service deliverables | ||
| For technical questions regarding capture array or next generation technology and the applications thereof, please email our next gen technical support. |
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