SeqWright -Next Gen DNA Sequencing

	Next Generation Sample Preparation Guidelines The following guidelines are designed to give you a basic idea of how to prepare your samples prior to sending us your next generation project. By following these guidelines, you will help us produce the best possible quantity and quality of data for you. However, not every application can be covered here and each project is different so, please contact us for your free project-specific criteria.
	SOLiD Sample Preparation
	Fragment Library-based Projects: DNA must be double-stranded Fragments must be > 500bp Avoid whole genome amplification methods whenever possible For fragment libraries of low complexity organisms, we recommend submitting 1-4µg of DNA at concentration of 100ng/µl For fragment libraries of high complexity organisms, we recommend submitting 4-20µg of DNA or more, at a concentration of 500ng/µl DNA should have an OD260/280 ratio of 1.8 or more Please provide a 1-2% agarose gel image of your sample with a 1kb ladder DNA should not be degraded, nor should it contain any particulate matter Mate-paired Library-based Projects: DNA must be double-stranded Avoid whole genome amplification methods whenever possible For mate-paired libraries of low complexity organisms, we recommend submitting 10-20µg of DNA at concentration of 1µg/µl For mate-paired libraries of high complexity organisms, we recommend submitting 50-100µg of DNA or more, at a concentration of 1µg/µl DNA should have an OD260/280 ratio of 1.8 or more Please provide a 1-2% agarose gel image of your sample with a 1kb ladder DNA should not be degraded, nor should it contain any particulate matter Transcriptome or small RNA-based Projects: Please contact SeqWright for guidelines
	If you are unable to meet these sample requirements, please contact a SeqWright representative for suggestions and technical support.

Next Generation Sample Preparation Guidelines