Clinical Trial Services
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Next Gen Sequencing
Capture Array

Process & Deliverables
Sample Preparation
DNA Sequencing
Molecular Genetics and Genomics
Microarray
 
Personal Genomic Profiling Service
 

 

 

Capture Array Process & Deliverables

 

 
Overview
The following steps are designed to provide you with a basic overview of SeqWright's genomic enrichment process. All, but the first two steps apply to exome capture projects as well.
 

How the process works (custom arrays)

  • Specify your region/s of interest (up to 30MB/array)
  • A custom array is designed and synthesized for your target gDNA
  • Send us your full-complexity human genomic DNA
  • We fragment your DNA (to 500bp) and hybridize it to your custom array
  • Unbound (undesired) DNA is washed away
  • The target DNA is then eluted and subsequently amplified
  • Following enrichment QC, the sample is ready for next gen sequencing
  
Deliverables

Upon Completion of your project, SeqWright will provide you with the following:

  • >6ug of enriched and amplified DNA (sufficient for multiple next-gen runs)
  • Report describing your enrichment yield and quality control metrics
  • Target region GFF adn BED file coverage summary
See our capture array sample preparation guidelines
     
  For technical questions regarding capture array or next generation technology and the applications thereof, please email our next gen technical support.