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Custom Molecular Biology & Cloning Services
Complementing our DNA sequencing services, SeqWright offers a broad range of custom molecular biology and subcloning services. All constructs are confirmed by DNA sequencing. Client may optionally receive all remaining intermediary products. Our experience includes, but is not limited to:
- Cloning/subcloning from gDNA, mRNA, BACs, PACs and other large constructs
- N-terminal and C-terminal tag integration
- Constructs for IRES or PBS directed multi-gene expression
- Promoter-reporter cloning and mutagenesis for promoter function analysis
- Any kind of vector switch, enzyme site adoption and multiclonal site changes
- Very high-throughput cloning strategies utilizing Gateway® technology
- PCR product cloning into T/A and TOPO vectors
- siRNA expression vector design and construction
- Construction of recombinant vectors (adenovirus, baculovirus, retrovirus, etc.)
- Custom mutagenesis services
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