Genomic Shotgun Library Construction & Sequencing

SeqWright has the capacity to manufacture over 300 custom shotgun libraries a month and can prepare libraries from a variety of samples including large genomes, adenoviral vectors, BACs, cosmids and more. All genomic libraries are constructed in-house within our GLP-compliant facility.

Standard Genomic Libraries

SeqWright is the exclusive supplier of custom shotgun libraries prepared by the 'Double Adaptor' method, utilized by BCM-HGSC in completing the Human Genome Project.

Double adapter shotgun library construction
Plasmid pWright™ vector capable of holding inserts up to 10kb (typically 1-3kb)
High percentage of clones carry inserts
No chimerics
Libraries submitted to customer as plates, glycerol stocks, or purified DNA
QC by DNA sequencing to ensure clones carry inserts
Construction of libraries in custom plasmid vector libraries available
gDNA preparation available
2 week turnaround time in most cases

Whole Genome Shotgun Sequencing

Automated picking and arraying colonies into 96- or 384-well plate format
Robotic DNA preparation
High-throughput sequencing using ABI Prism™ 3730xl sequencers
Contig assembly, mapping and gap closure
Geospiza Finch® Suite sample tracking sample tracking

More Library Construction Options

cDNA (more info)
Lambda
Cosmid
BAC