Random (Saturation) Mutagenesis Libraries

SeqWright can construct mutant libraries of specific genes through random (saturation) mutagenesis. The mutations are introduced through the use of certain DNA polymerases that are prone to errors in replication. The customer can select the frequency of the mutations and the vector for the subcloning of their products. A random selection of clones will be sequenced to verify the presence and frequency of mutations.

Library generation of unexpected mutants encoding for proteins with a wide range of interesting and useful properties
Subcloning into any vector system
Mutation frequency confirmed by DNA sequencing
Large-scale DNA sequencing available