Site-Directed Mutagenesis

SeqWright can introduce any mutation, including single- or multiple-site deletions, insertions, and substitutions, into a gene of interest. Simply send us your clone and a list of the mutations you want. Turnaround time is minimal, so you can spend more time focusing on your expression studies and less on the busy work.

QC by DNA sequencing to confirm template prior to mutagenesis
Design of mutagenic oligonucleotide primers
PCR amplification
Transformation, culturing and DNA preparation
Sequence verification of random clones to confirm mutation
Rapid turnaround for a single-site mutation
Client receives glycerol stock and purified plasmid DNA
Subcloning in multiple expression vectors optional
References are available