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Retrovirus/Lentivirus
- RNA extraction and QC on an Agilent 2100 Bioanalyzer RNA LabChip and a NanoDrop ND-1000
- Site-specific reverse transcription (random hexamer optional)
- PCR and subcloning
- Multiple full-length clones selected for sequencing
- Complete double-strand coverage obtained from multiple subclones
- Sequencing includes R & U5
Plasmid
- DNA preparation
- Full-length DS-sequencing coverage
- Primer walking or shotgun methodology
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