siRNA Expression Vector Construction
Tell us your target , and we'll do the rest. SeqWright will help you solve the two major tasks for using siRNA knockdown: selecting the best target sites and vector for delivering siRNAs into cells. We also offer recombinant virus production services for in vivo use or for cells that are difficult to transfect.
Target Site Selection Services
- siRNA expression cassette production
- Vector construction for GeneEraser™ Luciferase Suppression Test System
- Optimal target site identification from published literature
- siRNA expression vector construction (4 targets provided for selecting best knockdown)
Available siRNA Vector Types
- Prokaryotic plasmids
- Eukaryotic plasmids for establishment of stable cell lines
- Recombinant adenovirus systems
- Recombinant retrovirus systems (including lentivirus)
- Inducible siRNA expression systems
Novel Knockdown Strategies
- Double Cassette Systems: Different promoters and different target regions for enhanced synergistic knockdown
- Loss-of-function approaches employing siRNA and dominant-negative mutant proteins
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