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Custom Subcloning Service
Subcloning is easier when someone else does it. Clients need only provide the starting template and reference sequence file. Let SeqWright's scientist complete the rest. Our subcloning strategies may include any of the following steps:
- Restriction digest and gel purification of the target fragment
- Insert generation via PCR
- Custom designed synthetic insert construction (gene synthesis)
- DNA fragment modification (linkers, tags, etc.)
- Insert ligation into the vector of choice
- Midi- and maxi-preps available
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