Genotyping of transgenic mice shortly after birth drastically reduces the costs of strain maintenance. The speed and price of performing such genotyping is especially important when working with large and multiple litters. SeqWright offers a fast and convenient service for large-scale mouse genotyping which fits into any research budget. Submit tail biopsies or ear punches. PCR, QPCR and Southern hybridization assays are available. Probe sets may be provided by the investigator or designed by SeqWright where they may be maintained for future studies.
| |
| Lane No. |
Sample No. |
WT |
Targeted |
Cre Excised |
| 1 |
1068cav-10 |
+ |
+ |
n/a |
| 2 |
1068cav-11 |
+ |
- |
n/a |
| 3 |
1068cav-12 |
+ |
- |
n/a |
| 4 |
1068cav-13 |
+ |
+ |
n/a |
| 5 |
1068cav-14 |
+ |
- |
n/a |
| 6 |
1068cav-15 |
+ |
+ |
n/a |
| 7 |
1068cav-16 |
+ |
- |
n/a |
| 8 |
1068cav-17 |
+ |
+ |
n/a |
| 9 |
1068cav-18 |
+ |
+ |
n/a |
| 10 |
1068cav-19 |
+ |
- |
n/a |
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| |
| Service Features |
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• |
Assay design for genotyping specific transgenic events |
• |
Probe manufacturing |
• |
DNA extraction from tail biopsies, ear punches and other tissues |
• |
Genotyping by PCR, QPCR or hybridization (Southern blot) |
• |
Scalable to very large batches |
• |
Report included |
• |
3 day turnaround |
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| |
| Additional Resources |
• |
Genetic Stability Testing (GST) using QPCR and Southern blot analysis (info) |
• |
QPCR services (info) |
• |
DNA and tissue banking for transgenic samples |
• |
Contact a sales representative about this this service (contact info ) |
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