DNA Mutagenesis Services || SeqWright

The typical mutagenesis project for research scientists performing the whole process themselves goes something like this:

•	Sequence confirm cDNA clone to make sure you have the correct clone (pay for sequencing cost plus shipping)
•	Design and synthesize mutagenic oligonucleotide primers (pay for oligo synthesis and shipping)
•	Perform PCR, transformation, DNA culturing, and preparation
•	Sequence multiple clones to find just 1 with the right mutation (pay for sequencing all these clones plus shipping)
•	Hope that after all this work that you have the right clone, else send more clones out for sequencing, or restart the process

SeqWright takes care of this whole process for you, affordably, to your exact specifications, and in minimal time.

SAVE TIME & MONEY

Site-Directed Mutagenesis

SeqWright can introduce any mutation, including single- or multiple-site deletions, insertions, and substitutions, into a gene of interest. Simply send us your clone and a list of the mutations you want. Turnaround time is minimal, so you can spend more time focusing on your expression studies and less on the busy work.


•	QC by DNA sequencing to confirm template prior to mutagenesis
•	Design of mutagenic oligonucleotide primers
•	PCR amplification
•	Transformation, culturing and DNA preparation
•	Sequence verification of random clones to confirm mutation
•	Rapid turnaround for a single-site mutation
•	Client receives glycerol stock and purified plasmid DNA
•	Subcloning in multiple expression vectors optional
•	References are available

Site-specific primers introduce the desired mutations.

Thermal cycling amplifies the plasmid.

Amplified products contain desired mutation.

The mutated plasmid is isolated and transformed into cells.

Random (Saturation) Mutagenesis Libraries

SeqWright can construct mutant libraries of specific genes through random (saturation) mutagenesis. The mutations are introduced through the use of certain DNA polymerases that are prone to errors in replication. The customer can select the frequency of the mutations and the vector for the subcloning of their products. A random selection of clones will be sequenced to verify the presence and frequency of mutations.


•	Library generation of unexpected mutants encoding for proteins with a wide range of interesting and useful properties
•	Subcloning into any vector system
•	Mutation frequency confirmed by DNA sequencing
•	Large-scale DNA sequencing available

Additional Resources

•	cDNA synthesis, library sequencing and related services (info)
•	Custom gene synthesis and vector construction (info)
•	Relative and absolute quantitation with QPCR (info)
•	Serial Analysis of Gene Expression (SAGE) DNA sequencing (info)
•	Microarray expression analysis LInk to our affy page
•	Contact a sales representative about this service (contact info)