Ordering & Shipping Information || SeqWright

How do I get started?
∴	Get a Quotation for Your Project Request New customers should contact SeqWright for pricing information. For standard DNA sequencing services, quotations are typically issued for ongoing project submissions for up to one year. Specialty projects such as mutagenesis, subcloning and QPCR will be quoted on an ad hoc basis.
∴	Complete an Ordering Form (INFO)
∴	Sample Submission & Shipping Requirements (INFO)
∴	Retrieval of Results DNA sequencing results are made available via email or secure (password protected) web download, typically 24 hours after samples have arrived at SeqWright. Customers may select their data retrieval preferences on their ordering forms.
∴	Contact/Shipping Information (INFO)

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Ordering Forms
Download any one of the following ordering forms based on your project type. All forms should be emailed to seq@seqwright.com in advance and MUST BE submitted as a hardcopy with every package shipped to SeqWright.

∴	Standard Single-Tube DNA Sequencing Form (DOWNLOAD)
∴	96-Well Plate DNA Sequencing Form in MS Excel Format (DOWNLOAD)
∴	siRNA Expression Vector Construction Form (DOWNLOAD)
∴	qPCR Services Form (DOWNLOAD)
∴	microbial ID Form (DOWNLOAD)
∴	microarray Form (DOWNLOAD)
∴	Generic Customer Information Form (DOWNLOAD) Some projects are so customized that they do not fit into any one of the form categories listed above. If you think your project is one of these, please use this generic ordering form. SeqWright must have a form submitted with every package that enters our facility for appropriate sample handling and processing. It is also recommended to include a printed copy of your quotation as supplemental information.

YOU MUST SUBMIT A COMPLETED ORDER FORM WITH EVERY PACKAGE FOR PROPER INDENTIFICATION, TRACKING, AND SAMPLE HANDLING

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Sample Submission Requirements

For the best results, please follow these requirements:

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QC Your DNA
The #1 reason for poor sequencing results is that researchers do not take the time to QC their samples, whether by OD or agarose gel. Simply performing a mini-prep is not sufficient as it does not guarantee the appropriate concentration and yield for sequencing. Please verify your sample quality and amounts before submitting.

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DNA and Primer Sent Separately

SeqWright does not require premixing of DNA and primer for submissions. The main benefits of not premixing are:

•	Customers may request additional reactions to be performed on a particular DNA template without having to ship more sample
•	SeqWright can troubleshoot projects, such as adjusting DNA-primer concentration or choosing alternate primer sites in the event of poor sequencing quality following first round attempts
•	Template purification or amplification may be performed if necessary

For submitting DNA and primer separately, please refer to the following table:

Template	Concentration	Volume / RXN
Plasmid	50 ng/µL	10µL
PCR < 500	20-30 ng/µL	10µL
PCR > 500	30-50 ng/µL	10µL
Primer	5µM	10µL

PLEASE NOTE: Typically we request more DNA than we need in order to have a sufficient quantity for any repeats that we may need to perform. In addition, a researcher may get different sample concentration values depending on his/her quantitation method.

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DNA & Primer Combined
For sending combined samples, please refer to the following chart:

DNA Type	Size	DNA Conc. in H2O	DNA Volume	Total Amount	Primer Conc.	Primer Vol.
Plasmid	<6kb	40-60 ng/µl	8 µl	~500 ng	2 pmole/µl	4 µl
Plasmid	6-10kb	60-80 ng/µl	8 µl	~600 ng	2 pmole/µl	4 µl
Plasmid	>10kb	80-100 ng/µl	8 µl	~800 ng	2 pmole/µl	4 µl
PCR Product	<1kb	5-15 ng/µl	8 µl	~80 ng	2 pmole/µl	4 µl
PCR Product	1-2kb	~20 ng/µl	8 µl	~150 ng	2 pmole/µl	4 µl

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Standard Single-Tube DNA Sequencing (Purified Plasmids & PCR Products)
Please submit DNA and primers at volumes/concentrations according to the chart above. We do not accept PCR strip tubes for our single-tube service; only standard 1.5 mL bullet tubes or 2 mL screw cap tubes. Each tube must be clearly labeled with the sample's identity. We recommend putting your tubes in a larger 50 mL tube for added protection during shipment. DNA should be shipped overnight (next day delivery) at room temperature.

Acceptable Container Types	Shipping Conditions	Comments
1.5 mL Bullet Tube; 2mL Screw Cap Tubes	Overnight Mail @ Room Temperature	No PCR Strip Tubes

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96-Well Plate DNA Sequencing (Purified Plasmids & PCR Products)
Please submit DNA and primers at volumes/concentrations according to the chart above. Plates must be arrayed A1-H1, A2-H2...etc. as follows:

It is very important that plates are sealed and shipped FROZEN on dry ice.

Acceptable Container Types	Shipping Conditions	Comments
96-Well Semi-Skirted PCR Plates; 384-Well PCR Plates	Overnight Mail; Cooler; Dry Ice	No PCR Strip Tubes

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qPCR Sample Requirements:

Quantity	Solution	Concentration
2-3 ug DNA/RNA	Water or TE Buffer	250 ng/ul

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Other Common Sample Types:

Template Type	Shipping Conditions
Glycerol Stocks	Frozen; Dry Ice
Colonies/Agar Plates	Room Temperature; Petri Dish Sized Plates Only
Libraries (Transformation & Ligation Mixtures)	Frozen; Dry Ice
RNA	Frozen; Dry Ice

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Contact/Shipping Information

∴	Shipping Address SeqWright, Inc. Attn: New Order Request 2575 West Bellfort Suite 2001 Houston, TX 77054
∴	Customer Service and General Inquires U.S. Customers: 800-720-GENE (4363) International: 713-528-GENE (4363) Email: seq@seqwright.com
∴	Sales and Quotation Requests U.S. Customers: 800-720-GENE (4363) International: 713-528-GENE (4363) Email: sales@seqwright.com
∴	Audit Scheduling/Quality Assurance Department U.S. Customers: 800-720-GENE (4363) International: 713-528-GENE (4363) Email: quality@seqwright.com
∴	Next Generation SOLiD Support U.S. Customers: 800-720-GENE (4363) International: 713-528-GENE (4363) Email: solidSupport@seqwright.com