QPCR Services - Absolute, Relative & Allelic Discrimination || SeqWright

•	Copy number determination of DNA or RNA targets
•	Allelic discrimination / SNP genotyping
•	Gene expression profiling (siRNA knockdown & microarray study validation)
•	microRNA analysis
•	Plus/minus assays
•	Microbial detection and residual DNA analysis
•	Biodistribution studies of gene therapy products
•	Transgenic mouse genotyping
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	Download our qPCR ordering form
	See our qPCR sample requirements

Real-time quantitative PCR remains one of the most sensitive and quantitative tools for gene expression used today. SeqWright offers both custom and pre-optimized QPCR assays for an assortment of specific applications. We manage all aspects of the project from assay design, to nuceic acid isolation, to data analysis.

Service Features and Options

•	qPCR primer and probe design
•	Nucleic acid isolation from tissue, cells, biotechnology products, etc.
•	Quantitation using a NanoDrop® ND-1000
•	Qualitative analysis by an Agilent BioAnalyzer®
•	Primer/Probe optimization
•	Samples assayed in duplicate to ensure reproducibility
•	Controls included on each thermocycling run
•	Data report with project design description
•	Statistical analysis upon request

microRNA Services

From isolation of small RNA’s to downstream analysis of their expression level, just send us your samples and we can do the rest. miRNA's offer insight into a complex world of gene regulation that is just beginning to come to light. Let our RNA Scientists and Bioinformatic experts help you get the most out of your miRNA experiments.

SeqWright can perform microRNA Analysis on the following Arrays

TaqMan® microRNA Assays, Human	TaqMan® microRNA Assays, Arabidopsis thaliana
TaqMan® microRNA Assays, Mouse	TaqMan® microRNA Assays, Caenorhabditis elegans
TaqMan® microRNA Assays, Rat	TaqMan ® microRNA Assays, DrosophilaMelanogaster

Additional Resources

•	FDA submission support services (info)
•	Custom siRNA services (info)
•	cGMP microbial identification service for biosterility QC (info)
•	Standard microbial identification via 16s sequencing (info)
•	Transgenic mouse genotyping services (info)
•	Gene transfer vector DNA sequencing (info)
•	Microarray expression analysis