Shipping Information

Shipping Address
SeqWright, Inc.
Attn: New Order Request
2575 West Bellfort
Suite 2001
Houston, TX 77054

Sample Submission Requirements
For the best results, please follow these requirements:

QC Your DNA
The #1 reason for poor sequencing results is that researchers do not take the time to QC their samples, whether by OD or agarose gel. Simply performing a mini-prep is not sufficient as it does not guarantee the appropriate concentration and yield for sequencing. Please verify your sample quality and amounts before submitting.

DNA and Primer Sent Separately
SeqWright does not require premixing of DNA and primer for submissions. The main benefits of not premixing are:

Customers may request additional reactions to be performed on a particular DNA template without having to ship more sample
SeqWright can troubleshoot projects, such as adjusting DNA-primer concentration or choosing alternate primer sites in the event of poor sequencing quality following first round attempts
Template purification or amplification may be performed if necessary

For submitting DNA and primer separately, please refer to the following table:

Template	Concentration	Volume / RXN
Plasmid	50 ng/µL	10µL
PCR < 500	20-30 ng/µL	10µL
PCR > 500	30-50 ng/µL	10µL
Primer	5µM	10µL

PLEASE NOTE: Typically we request more DNA than we need in order to have a sufficient quantity for any repeats that we may need to perform. In addition, a researcher may get different sample concentration values depending on his/her quantitation method.

DNA & Primer Combined
For sending combined samples, please refer to the following chart:

DNA Type	Size	DNA Conc. in H2O	DNA Volume	Total Amount	Primer Conc.	Primer Vol.
Plasmid	<6kb	40-60 ng/µl	8 µl	~500 ng	2 pmole/µl	4 µl
Plasmid	6-10kb	60-80 ng/µl	8 µl	~600 ng	2 pmole/µl	4 µl
Plasmid	>10kb	80-100 ng/µl	8 µl	~800 ng	2 pmole/µl	4 µl
PCR Product	<1kb	5-15 ng/µl	8 µl	~80 ng	2 pmole/µl	4 µl
PCR Product	1-2kb	~20 ng/µl	8 µl	~150 ng	2 pmole/µl	4 µl

Standard Single-Tube DNA Sequencing (Purified Plasmids & PCR Products)
Please submit DNA and primers at volumes/concentrations according to the chart above. We do not accept PCR strip tubes for our single-tube service; only standard 1.5 mL bullet tubes or 2 mL screw cap tubes. Each tube must be clearly labeled with the sample's identity. We recommend putting your tubes in a larger 50 mL tube for added protection during shipment. DNA should be shipped overnight (next day delivery) at room temperature.

Acceptable Container Types	Shipping Conditions	Comments
1.5 mL Bullet Tube; 2mL Screw Cap Tubes	Overnight Mail @ Room Temperature	No PCR Strip Tubes

96-Well Plate DNA Sequencing (Purified Plasmids & PCR Products)
Please submit DNA and primers at volumes/concentrations according to the chart above. Plates must be arrayed A1-H1, A2-H2...etc. as follows:

It is very important that plates are sealed and shipped FROZEN on dry ice.

Acceptable Container Types	Shipping Conditions	Comments
96-Well Semi-Skirted PCR Plates; 384-Well PCR Plates	Overnight Mail; Cooler; Dry Ice	No PCR Strip Tubes

qPCR Sample Requirements:

Quantity	Solution	Concentration
2-3 ug DNA/RNA	Water or TE Buffer	250 ng/ul

Other Common Sample Types:

Template Type	Shipping Conditions
Glycerol Stocks	Frozen; Dry Ice
Colonies/Agar Plates	Room Temperature; Petri Dish Sized Plates Only
Libraries (Transformation & Ligation Mixtures)	Frozen; Dry Ice
RNA	Frozen; Dry Ice